Effect of Hormone-treatment Duration in <i>in vitro </i>Maturation of Immature Oocytes on Pig Cloning Efficiency

Chang Sin Rim; Yu Song Kim; Yong Je Ri; Ryu Chol Kim; Chang Gon Sin; Chol Ho Rim; Kwang Il To; Ui Myong Jong

doi:doi:10.11648/j.reports.20250504.12

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Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency

Chang Sin Rim, Yu Song Kim, Yong Je Ri, Ryu Chol Kim, Chang Gon Sin, Chol Ho Rim, Kwang Il To^*

, Ui Myong Jong

Published in Reports (Volume 5, Issue 4)

Received: 26 September 2025 Accepted: 18 October 2025 Published: 28 November 2025

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Abstract

This study aims to analyze the influence of hormone-treatment duration in in vitro maturation of porcine immature oocytes on the pig cloning efficiency. Although there were no significant differences in the maturation rate of immature oocytes and cleavage rate of somatic cell nuclear transfer embryos according to the hormone-treatment period (22, 27, 32, 37 and 42 h), the blastocyst rates of somatic cell nuclear transfer embryos derived from oocytes cultivated for 22, 27 and 32 h (19.0%, 21.5% and 22.4%, respectively) were significantly (P<0.05) higher than those for 37 and 42 h (13.2% and 12.4%, respectively). In particular, there were significant (P<0.05) increase in the hatching blastocyst rates of 27 and 32 h cultivated groups (13.7% and 16.5%, respectively) compared to 22, 37, 42h cultivated groups (8.2%, 5.6% and 6.7%, respectively). Moreover, the cloning efficiency and the delivery rates of normal lives of 27 (1.4% and 1.2%, respectively) and 32h (1.5% and 1.3%, respectively) cultivated groups were significantly (P<0.05) higher than those of 22h cultivated group (0.9% and 0.6%, respectively). To sum up, this study shows that the increase of hormone-treatment duration in in vitro maturation of porcine immature oocytes to a certain extent has a positive impact on the cloning efficiency.

Published in	Reports (Volume 5, Issue 4)
DOI	10.11648/j.reports.20250504.12
Page(s)	65-71
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

In vitro Maturation, Hormone, Nuclear Transfer, Cloning, Pig

References

[1]	Shi, J. S., Zhou, R., Luo, L. H., Mai, R. B., Zeng, H. Y., He, X. Y., Liu, D. W., Zeng, F., Cai, G. Y., Ji, H. M., Tang, F., Wang, Q. L., Wu, Z. F., Li, Z. C., 2015. Influence of embryo handling and transfer method on pig cloning efficiency. Anim. Reprod. Sci. 154, 121–127. https://doi.org/10.1016/j.anireprosci.2015.01.006
[2]	Kawarasaki, T., Enya, S., Otake, M., Shibata, M., Mikawa, S., 2017. Reproductive performance and expression of imprinted genes in somatic cell cloned boars. Anim. Sci. J. 88, 1801–1810. https://doi.org/10.1111/asj.12838
[3]	Shi, J., Tan, B., Luo, L., Li, Z., Hong, L., Yang, J., Cai, G., Zheng, E., Wu, Z., Gu, T., 2020. Assessment of the growth and reproductive performance of cloned pietrain Boars. Animals 10. https://doi.org/10.3390/ani10112053
[4]	Galli, C., Lazzari, G., 2021. 25th anniversary of cloning by somatic-cell nuclear transfer: current applications of SCNT in advanced breeding and genome editing in livestock. Reproduction 162, F23–F32. https://doi.org/10.1530/REP-21-0006
[5]	Polejaeva, I. A., 2021. 25th anniversary of cloning by somatic cell nuclear transfer: generation of genetically engineered livestock using somatic cell nuclear transfer. Reproduction 162, F11–F22. https://doi.org/10.1530/rep-21-0072
[6]	Mir, B., Zaunbrecher, G., Archer, G. S., Friend, T. H., Piedrahita, J. A., 2005. Progeny of somatic cell nuclear transfer (SCNT) pig clones are phenotypically similar to non-cloned pigs. Cloning Stem Cells 7, 119–125. https://doi.org/10.1089/clo.2005.7.119
[7]	Pan, D., Zhang, Y., Sun, X., Zhang, J., Li, X., Li, Y., Gu, Z., Dai, Y., Wu, C., Li, N., 2006. Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension. Chin. Sci. Bull. 51, 839–844. https://doi.org/10.1007/s11434-006-0839-4
[8]	Chen, C. H., Jiang, B. H., Huang, S. Y., Yang, T. S., Lee, K. H., Tu, C. F., Wu, S. C., 2013. Genetic polymorphisms, growth performance, hematological parameters, serum enzymes, and reproductive characteristics in phenotypically normal Landrace boars produced by somatic cell nuclear transfer. Theriogenology 80, 1088–1096. https://doi.org/10.1016/j.theriogenology.2013.08.010
[9]	Adachi, N., Yamaguchi, D., Watanabe, A., Miura, N., Sunaga, S., Oishi, H., Hashimoto, M., Oishi, T., Iwamoto, M., Hanada, H., Kubo, M., Onishi, A., 2014. Growth, reproductive performance, carcass characteristics and meat quality in F1 and F2 progenies of somatic cell-cloned pigs. J. Reprod. Dev. 60, 100–105. https://doi.org/10.1262/jrd.2012-167
[10]	Gu, T., Shi, J., Luo, L., Li, Z., Yang, J., Cai, G., Zheng, E., Hong, L., Wu, Z., 2019. Study on hematological and biochemical characters of cloned duroc pigs and their progeny. Animals 9. https://doi.org/10.3390/ani9110912
[11]	Shengyi, Mao, Zhuo, Li, Hua, Kui, Deling, J., Yuying, Li, Yubo, Q., Jianxiong, Guo, Yunfang, Wei, Hongjiang, Wei, 2020. Application of somatic cell cloning technology in the protection of genetic resources of Wujin pigs. Acta Vet. Et. Zootech. Sin. 51, 2130–2137.
[12]	Fraguas-Eggenschwiler, M., Eggenschwiler, R., Sollner, J. H., Cortnumme, L., Vondran, F. W. R., Cantz, T., Ott, M., Niemann, H., 2021. Direct conversion of porcine primary fibroblasts into hepatocyte-like cells. Sci. Rep. 11, 9334. https://doi.org/10.1038/s41598-021-88727-1
[13]	Otake, M., Kawaguchi, H., Enya, S., Kangawa, A., Koga, T., Matsuo, K., Yamada, S., Rahman, M. M., Miura, N., Shibata, M., Tanimoto, A., 2021. High pathological reproducibility of diet-induced atherosclerosis in micro minipigs via cloning technology. Vivo 35, 2025–2033. https://doi.org/10.21873/invivo.12471
[14]	Junsong Shi, Liyao Xiao, Baohua Tan, Lvhua Luo, Zicong Li, Linjun Hong, Jie Yang, Gengyuan Cai, Enqin Zheng, Zhenfang Wu, Ting Gu, 2022. Comparative evaluation of production performances of cloned pigs derived from superior Duroc boars. Anim. Reprod. Sci. 244, 107049. https://doi.org/10.1016/j.anireprosci.2013.10.004
[15]	Marchal, R., Feugang, J. M., Perreau, C., Venturi, E., Terqui, M., Mermillod, P., 2001. Meiotic and developmental competence of prepubertal and adult swine oocytes. Theriogenology 56, 17-29. https://doi.org/10.1016/S0093-691X(01)00539-8
[16]	Lin, P., Rui, R., 2010. Effects of follicular size and FSH on granulosa cell apoptosis and atresia in porcine antral follicles. Mol. Reprod. Dev. 77, 670–678. https://doi.org/10.1002/mrd.21202
[17]	Matsuda, F., Inoue, N., Manabe, N., Ohkura, S., 2012. Follicular growth and atresia in mammalian ovaries: regulation by survival and death of granulosa cells. J. Reprod. Dev. 58, 44–50. https://doi.org/10.1262/jrd.2011-012
[18]	Samiec, M., Skrzyszowska, M., 2012. High developmental capability of porcine cloned embryos following trichostatin A-dependent epigenomic transformation during in vitro maturation of oocytes pre-exposed to R-roscovitine. Anim. Sci. Pap. Rep. 30(4), 383-393.
[19]	Lonergan, P., Fair, T., 2014. The ART of studying early embryo development: Progress and challenges in ruminant embryo culture. Theriogenology 81, 49–55. https://doi.org/10.1016/j.theriogenology.2013.09.021
[20]	Pedersen, H. S., Callesen, H., Lovendahl, P., Chen, F., Nyengaard, J. R., Nikolaisen, N. K., Holm, P., Hyttel, P., 2016. Ultrastructure and mitochondrial numbers in pre and post pubertal pig oocytes. Reprod. Fertil. Dev. 28, 586–598. https://doi.org/10.1071/RD14220
[21]	Yuan, Y., Spate, L. D., Redel, B. K., Tian, Y., Zhou, J., Prather, R. S., 2017. Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation. Proc Natl Acad Sci Unit States Am 114, E5796-804. https://doi.org/10.1073/pnas.1703998114
[22]	Wang, H., Cui, W., Meng, C., Zhang, J., Li, Y., Qian, Y., Xing, G., Zhao, D., Cao, S., 2018. MC1568 enhances histone acetylation during oocyte meiosis and improves development of somatic cell nuclear transfer embryos in pig. Cell. Reprogram. 20(1), 55-65. https://doi.org/10.1089/cell.2017.0023
[23]	Gupta, M. K., Heo, Y. T., Kim, D. K, Lee, H. T., Uhm, S. J., 2019. 5-Azacytidine improves the meiotic maturation and subsequent in vitro development of pig oocytes. Anim. Reprod. Sci. 208, 106118 https://doi.org/10.1016/j.anireprosci.2019.106118
[24]	He, X., Tan, C., Li, Z., Zhao, C., Shi, J., Zhou, R., Wang, X., Jiang, G., Cai, G., Liu, D., Wu, Z., 2019. Characterization and comparative analyses of transcriptomes of cloned and in vivo fertilized porcine pre-implantation embryos. Biol. Open 8. https://doi.org/10.1242/bio.039917
[25]	Kito, S., Bavister, B. D., 1997. Male pronuclear formation and early embryonic development of hamster oocytes matured in vitro with gonadotrophins, amino acids and cysteamine. J. Reprod. Fertil. 110, 35–46.
[26]	Downs, S. M., Mastropolo, A. M., 1997. Culture conditions affect meiotic regulation in cumulus cell-enclosed mouse oocytes. Mol. Reprod. Dev. 46, 551–566.
[27]	Accardo, C., Dattena, M., Pilichi, S., Mara, L., Chessa, B., Cappai, P., 2004. Effect of recombinant human FSH and LH on in vitro maturation of sheep oocytes; embryo development and viability Ani. Reprod. Sci. 81, 77–86. https://doi.org/10.1016/j.anireprosci.2003.10.004
[28]	Marques, M. G., Nicacio, A. C., Oliveira, V. P., Nascimento, A. B., Caetano, H. V. A., Mendes, C. M., Mello, M. R. B., Milazzotto, M. P., Assumpção, M. E. O. A., Visintin, J. A., 2007. In vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors. Anim. Rep. Sci. 97, 375–381. https://doi.org/10.1016/j.anireprosci.2006.02.013
[29]	Niemann, H., 2016. Epigenetic reprogramming in mammalian species after SCNT-based cloning. Theriogenology 86, 80–90. https://doi.org/10.1016/j.theriogenology.2016.04.021
[30]	Gioia, L., Festuccia, C., Colapietro, A., Alessia G., Contri, A., Valbonetti, L., 2019. Abundances of autophagy-related protein LC3B in granulosa cells, cumulus cells, and oocytes during atresia of pig antral follicles. Anim. Reprod. Sci. 211, 106225 https://doi.org/10.1016/j.anireprosci.2019.106225
[31]	Konno, S., Wakayama, S., Ito, D., Kazama, K., Hirose, N., Ooga, M., Wakayama, T., 2020. Removal of remodeling/reprogramming factors from oocytes and the impact on the full-term development of cloned embryos. Development, 147(15), dev190777. https://doi.org/10.1242/dev.190777
[32]	Younis, A. I., Brackett, B. G., Fayer-Hosken, R. A., 1989. Influence of serum and hormones on bovine oocyte maturation and fertilization in vitro. Gamete Res. 23, 189–201.
[33]	Thompson, J. G., 2000. In vitro culture and embryo metabolism of cattle and sheep embryos-a decade of achievement. Anim. Reprod. Sci. 30, 273–280.
[34]	Zuelke, K. A., Brackett, B. G., 1990. Luteinizing hormones enhanced in vitro maturation of bovine oocytes with and without protein supplementation. Biol. Reprod. 43, 784–787.
[35]	Moor, R. M., Trouson, A. O., 1997. Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J. Reprod. Fertil. 49, 101–109.
[36]	Inger Faerge, Frantisek Strejcek, Jozef Laurincik, Detlef Rath, Heiner Niemann, Karl Schellander, Christine Rosenkranz, Poul Maddox Hyttel, Christian Grøndahl, 2006. The effect of FF-MAS on porcine cumulus–oocyte complexmaturation, fertilization and pronucleus formation in vitro. Zygote 14, 189–199. https://doi.org/10.1017/S0967199406003765
[37]	Cayo-Colca, I. S., Yamagami, Y., Phan, T. C., Miyano, T., 2011. A combination of FSH and dibutyryl cyclic AMP promote growth and acquisition of meiotic competence of oocytes from early porcine antral follicles. Theriogenology 75, 1602–1612. https://doi.org/10.1016/j.theriogenology.2010.12.023
[38]	Evecen, M., Cirit, U., Demir, K., Hamzaoglu, A. I., Bakırera, G., Pabuccuoglu S., Birler S., 2011. Adding hormones sequentially could be an effective approach for IVM of dog oocytes Theriogenology 75, 1647–1651 https://doi.org/10.1016/j.theriogenology.2011.01.004
[39]	Kenichiro Sakaguchi, Masashi Nagano, 2020. Follicle priming by FSH and pre-maturation culture to improve oocyte quality in vivo and in vitro. Theriogenology 150, 122-129. https://doi.org/10.1016/j.theriogenology.2020.01.023
[40]	Richard, F. J., Sirard, M. A., 1996. Effects of follicular cells on oocyte maturation. Theca cell inhibition of bovine oocyte maturation in vitro. Biol. Reprod. 1, 22–28.
[41]	Niemann, H., and Rath, D., 2001. Progress in reproductive biotechnology in swine. Theriogenology 56, 1291–1304.
[42]	Ongaratto, F. L., Rodriguez-Villamil, P., Bertolini, M., Carlson, D. F., 2020. Influence of oocyte selection, activation with a zinc chelator and inhibition of histone deacetylases on cloned porcine embryo and chemically activated oocytes development. Zygote 28(4), 286-290. https://doi.org/10.1017/S0967199419000856
[43]	Matoba, S., Zhang, Y., 2018. Somatic cell nuclear transfer reprogramming: mechanisms and applications. Cell Stem Cell 23, 471–485. https://doi.org/10.1016/j.stem.2018.06.018
[44]	Hulinska P., Martecikova S., Jeseta M., Machatkova M., 2011. Efficiency of in vitro fertilization is influenced by the meiotic competence of porcine oocytes and time of their maturation Anim. Reprod. Sci. 124, 112–117. https://doi.org/10.1016/j.anireprosci.2011.02.004
[45]	Chengcheng, Zhao, Junsong, Shi, Rong, Zhou, Ranbiao, Mai, Lvhua, Luo, Xiaoyan, He, Hongmei, Ji, Gengyuan, Cai, Dewu, Liu, Enqin, Zheng, Zhenfang, Wu, Zicong, Li, 2019. Effects of enucleation method on in vitro and in vivo development rate of cloned pig embryos. Front. Agr. Sci. Eng. (6), 61–65. https://doi.org/10.15302/J-FASE-2018227
[46]	Funahashi, H., Day, B. N., 1993. Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro. J. Reprod. Fertil. 98, 424–431.

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Rim, C. S., Kim, Y. S., Ri, Y. J., Kim, R. C., Sin, C. G., et al. (2025). Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency. Reports, 5(4), 65-71. https://doi.org/10.11648/j.reports.20250504.12

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ACS Style

Rim, C. S.; Kim, Y. S.; Ri, Y. J.; Kim, R. C.; Sin, C. G., et al. Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency. Reports. 2025, 5(4), 65-71. doi: 10.11648/j.reports.20250504.12

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AMA Style

Rim CS, Kim YS, Ri YJ, Kim RC, Sin CG, et al. Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency. Reports. 2025;5(4):65-71. doi: 10.11648/j.reports.20250504.12

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@article{10.11648/j.reports.20250504.12,
  author = {Chang Sin Rim and Yu Song Kim and Yong Je Ri and Ryu Chol Kim and Chang Gon Sin and Chol Ho Rim and Kwang Il To and Ui Myong Jong},
  title = {Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency},
  journal = {Reports},
  volume = {5},
  number = {4},
  pages = {65-71},
  doi = {10.11648/j.reports.20250504.12},
  url = {https://doi.org/10.11648/j.reports.20250504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.reports.20250504.12},
  abstract = {This study aims to analyze the influence of hormone-treatment duration in in vitro maturation of porcine immature oocytes on the pig cloning efficiency. Although there were no significant differences in the maturation rate of immature oocytes and cleavage rate of somatic cell nuclear transfer embryos according to the hormone-treatment period (22, 27, 32, 37 and 42 h), the blastocyst rates of somatic cell nuclear transfer embryos derived from oocytes cultivated for 22, 27 and 32 h (19.0%, 21.5% and 22.4%, respectively) were significantly (PPP in vitro maturation of porcine immature oocytes to a certain extent has a positive impact on the cloning efficiency.},
 year = {2025}
}

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TY  - JOUR
T1  - Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency
AU  - Chang Sin Rim
AU  - Yu Song Kim
AU  - Yong Je Ri
AU  - Ryu Chol Kim
AU  - Chang Gon Sin
AU  - Chol Ho Rim
AU  - Kwang Il To
AU  - Ui Myong Jong
Y1  - 2025/11/28
PY  - 2025
N1  - https://doi.org/10.11648/j.reports.20250504.12
DO  - 10.11648/j.reports.20250504.12
T2  - Reports
JF  - Reports
JO  - Reports
SP  - 65
EP  - 71
PB  - Science Publishing Group
SN  - 2994-7146
UR  - https://doi.org/10.11648/j.reports.20250504.12
AB  - This study aims to analyze the influence of hormone-treatment duration in in vitro maturation of porcine immature oocytes on the pig cloning efficiency. Although there were no significant differences in the maturation rate of immature oocytes and cleavage rate of somatic cell nuclear transfer embryos according to the hormone-treatment period (22, 27, 32, 37 and 42 h), the blastocyst rates of somatic cell nuclear transfer embryos derived from oocytes cultivated for 22, 27 and 32 h (19.0%, 21.5% and 22.4%, respectively) were significantly (PPP in vitro maturation of porcine immature oocytes to a certain extent has a positive impact on the cloning efficiency.
VL  - 5
IS  - 4
ER  -

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Author Information

Chang Sin Rim

Department of Animal Cloning, Institute of Animal Genetic Technology, Pyongyang, DPR Korea
Yu Song Kim

Department of Animal Cloning, Institute of Animal Genetic Technology, Pyongyang, DPR Korea
Yong Je Ri

Department of Animal Cloning, Institute of Animal Genetic Technology, Pyongyang, DPR Korea
Ryu Chol Kim

Department of Animal Cloning, Institute of Animal Genetic Technology, Pyongyang, DPR Korea
Chang Gon Sin

Department of Animal Cloning, Institute of Animal Genetic Technology, Pyongyang, DPR Korea
Chol Ho Rim

Department of Animal Cloning, Institute of Animal Genetic Technology, Pyongyang, DPR Korea
Kwang Il To

Institute of Chemistry and Biology, University of Sciences, Pyongyang, DPR Korea

Contact Email

http://orcid.org/0009-0008-8481-5288
Ui Myong Jong

Department of Animal Cloning, Institute of Animal Genetic Technology, Pyongyang, DPR Korea

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Plain Text BibTeX RIS

APA Style

Rim, C. S., Kim, Y. S., Ri, Y. J., Kim, R. C., Sin, C. G., et al. (2025). Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency. Reports, 5(4), 65-71. https://doi.org/10.11648/j.reports.20250504.12

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ACS Style

Rim, C. S.; Kim, Y. S.; Ri, Y. J.; Kim, R. C.; Sin, C. G., et al. Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency. Reports. 2025, 5(4), 65-71. doi: 10.11648/j.reports.20250504.12

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AMA Style

Rim CS, Kim YS, Ri YJ, Kim RC, Sin CG, et al. Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency. Reports. 2025;5(4):65-71. doi: 10.11648/j.reports.20250504.12

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@article{10.11648/j.reports.20250504.12,
  author = {Chang Sin Rim and Yu Song Kim and Yong Je Ri and Ryu Chol Kim and Chang Gon Sin and Chol Ho Rim and Kwang Il To and Ui Myong Jong},
  title = {Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency},
  journal = {Reports},
  volume = {5},
  number = {4},
  pages = {65-71},
  doi = {10.11648/j.reports.20250504.12},
  url = {https://doi.org/10.11648/j.reports.20250504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.reports.20250504.12},
  abstract = {This study aims to analyze the influence of hormone-treatment duration in in vitro maturation of porcine immature oocytes on the pig cloning efficiency. Although there were no significant differences in the maturation rate of immature oocytes and cleavage rate of somatic cell nuclear transfer embryos according to the hormone-treatment period (22, 27, 32, 37 and 42 h), the blastocyst rates of somatic cell nuclear transfer embryos derived from oocytes cultivated for 22, 27 and 32 h (19.0%, 21.5% and 22.4%, respectively) were significantly (PPP in vitro maturation of porcine immature oocytes to a certain extent has a positive impact on the cloning efficiency.},
 year = {2025}
}

Copy | Download

TY  - JOUR
T1  - Effect of Hormone-treatment Duration in in vitro Maturation of Immature Oocytes on Pig Cloning Efficiency
AU  - Chang Sin Rim
AU  - Yu Song Kim
AU  - Yong Je Ri
AU  - Ryu Chol Kim
AU  - Chang Gon Sin
AU  - Chol Ho Rim
AU  - Kwang Il To
AU  - Ui Myong Jong
Y1  - 2025/11/28
PY  - 2025
N1  - https://doi.org/10.11648/j.reports.20250504.12
DO  - 10.11648/j.reports.20250504.12
T2  - Reports
JF  - Reports
JO  - Reports
SP  - 65
EP  - 71
PB  - Science Publishing Group
SN  - 2994-7146
UR  - https://doi.org/10.11648/j.reports.20250504.12
AB  - This study aims to analyze the influence of hormone-treatment duration in in vitro maturation of porcine immature oocytes on the pig cloning efficiency. Although there were no significant differences in the maturation rate of immature oocytes and cleavage rate of somatic cell nuclear transfer embryos according to the hormone-treatment period (22, 27, 32, 37 and 42 h), the blastocyst rates of somatic cell nuclear transfer embryos derived from oocytes cultivated for 22, 27 and 32 h (19.0%, 21.5% and 22.4%, respectively) were significantly (PPP in vitro maturation of porcine immature oocytes to a certain extent has a positive impact on the cloning efficiency.
VL  - 5
IS  - 4
ER  -

Copy | Download