The study aimed to determine the effect of limestone or extruded eggshells on the egg and eggshell quality of laying hens. A total of 240 and 60 eggs were collected from diets D1 (limestone) and D2 (extruded eggshells), respectively, with two levels of calcium source (L): L1 (4% limestone or eggshells) and L2 (8% limestone or eggshells) at 44, 52, 60, 68, and 72 weeks of age. These eggs were obtained from an ongoing project to measure egg quality and dry matter content. Diet D1 showed higher albumen width and height, Haugh unit, and a lower egg yolk height than D2 (p<0.05). However, the percentage of albumen and yolk weight, as well as the yolk index, tended to be higher in D2 than in D1. Other egg quality traits were similar between diets (p>0.05). The L2 was superior to L1 in both diets for egg quality traits (p<0.01), and the L2 in the extruded eggshell diet performed better than L2 in limestone diet. The egg, albumen, yolk, and eggshell weight, albumen width, and yolk height were increased but the eggshell strength, yolk color, albumen height, eggshell thickness, and membrane thickness were decreased with advancing the birds' age. Egg, albumen, yolk, and eggshell weight, as well as albumen width and yolk height, increased with the advancing age of the birds, whereas eggshell strength, yolk color, albumen height, eggshell thickness, and membrane thickness decreased. Egg, albumen, and eggshell with membrane weight were influenced by the diet × L interaction, while diet × age interaction affected other traits. Egg quality traits were influenced by L x A interaction, but were not affected by D x L x A interaction. The dry matter content of an egg was similar between diets, but moisture content was higher in D2 than in D1. The dry matter content of albumen was influenced by diet x age interaction. Therefore, the 8% extruded eggshell appears to be the most suitable diet for improving egg and eggshell quality.
| Published in | International Journal of Animal Science and Technology (Volume 9, Issue 2) |
| DOI | 10.11648/j.ijast.20250902.16 |
| Page(s) | 74-86 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Age, Ca Source Level, Dry Matter, Extruded Eggshell, Egg Quality, Laying Hen
| [1] | DLS. (2020). Livestock Economy at a Glance. Retrieved from DLS web portal: |
| [2] | El-Ishaq, A., & Kida, H. D., 2011. Comparative analysis of calcium carbonate content in eggshell of hen, duck and guinea fowl. IRAON, 3, 81-87. |
| [3] | Schaafsma, A., Pakan, I., Hofstede, G. J. H., Muskiet, F. A., Van Der Veer, E., & De Vries, P. J. F. (2000). Mineral, amino acid and hormonal composition of chicken eggshell powder and the evaluation of its use in human nutrition. Poultry Science, 79(12), 1833–1838. |
| [4] | Khosht, A. R., Abdel -Khalek, A. M. & Abed El-Magid, M. A. (2020). Influences of calcium levels with limestone particle size on laying performance, and eggshell quality traits of layers. Egyptian Poultry Science Journal, 40(3), 631-645. |
| [5] | Islam, A. (2020). The final report of the project “Use of extruded eggshell as a calcium source substituting limestone of oyster shell in the diet of laying hen”. Final Report, Ministry of Education, BANBES, Dhaka, Bangladesh. |
| [6] | Islam, M. A. & Nishibori, M. (2021). Use of extruded eggshell as a calcium source substituting limestone or oyster shell in the diet of laying hens. Veterinary Medicine and Science, 7, 1948-1958. |
| [7] |
Islam, M. A., Das, N. C., & Nishibori, M. (2022). Effect of extruded eggshell, limestone and oyster shell on egg production performance of laying hens. Journal of Agricultural and Crop Research, 10(3), 43-51.
http://sciencewebpublishing.net/jacr/archive/2022/June/pdf/Islam%20et%20al.pdf |
| [8] | Cufadar, Y. (2014). Effect of Alternative Calcium Sources on Performance and Eggshell Quality in Laying Hens. The Journal of Macro Trends in Applied Science, 21, 587-592. |
| [9] | Lichovnikova, M. (2007). The effect of dietary calcium source, concentration and particle size on calcium retention, eggshell quality and overall calcium requirement in laying hens. British Poultry Science, 48(1), 71–75. |
| [10] | Gongruttananun, N. (2011). Effects of using ground eggshells as a dietary calcium source on egg production traits, hatching performance and eggshell ultrastructure in laying hens. Kasetsart Journal. Natural Sciences, 45, 209-220. |
| [11] | Olgun, O., Yildiz, A. Ö., & Cufadar, Y. (2015). The effects of eggshell and oyster shell supplemental as calcium sources on performance, eggshell quality and mineral excretion in laying hens. Indian Journal of Animal Research, 49(2), 205. |
| [12] | Scheideler, S. E. (1998). Eggshell Calcium Effects on Egg Quality and Ca Digestibility in First- or Third-Cycle Laying Hens. Journal of Applied Poultry Research, 7(1), 69–74. |
| [13] | Sim, J. S., Aw-Yong, L. M., & Bragg, D. B. (1983). Utilization of eggshell waste by the laying hen. Poultry Science, 62, 2227-2229. |
| [14] | Chung, S. H. & Lee, K. W. (2014). Effect of Hen Age, Storage Duration and Temperature on Egg Quality in Laying Hens. International Journal of Poultry Science, 13(11), 634-636. |
| [15] | Padhi, M. K., Chatterjee R. N., Haunshi S., & Rajkumar, U. (2013). Effect of age on egg quality in chicken. Indian Journal of Poultry Science, 48(1), 122-125. |
APA Style
Islam, M. A., Devnath, D. D., Meem, I. J., Nishibori, M. (2025). Effect of Limestone or Extruded Eggshell on Egg and Eggshell Quality of Laying Hens. International Journal of Animal Science and Technology, 9(2), 74-86. https://doi.org/10.11648/j.ijast.20250902.16
ACS Style
Islam, M. A.; Devnath, D. D.; Meem, I. J.; Nishibori, M. Effect of Limestone or Extruded Eggshell on Egg and Eggshell Quality of Laying Hens. Int. J. Anim. Sci. Technol. 2025, 9(2), 74-86. doi: 10.11648/j.ijast.20250902.16
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijast.20250902.16,
author = {Mohammad Aminul Islam and Dibya Dipan Devnath and Israt Jahan Meem and Masahide Nishibori},
title = {Effect of Limestone or Extruded Eggshell on Egg and Eggshell Quality of Laying Hens
},
journal = {International Journal of Animal Science and Technology},
volume = {9},
number = {2},
pages = {74-86},
doi = {10.11648/j.ijast.20250902.16},
url = {https://doi.org/10.11648/j.ijast.20250902.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20250902.16},
abstract = {The study aimed to determine the effect of limestone or extruded eggshells on the egg and eggshell quality of laying hens. A total of 240 and 60 eggs were collected from diets D1 (limestone) and D2 (extruded eggshells), respectively, with two levels of calcium source (L): L1 (4% limestone or eggshells) and L2 (8% limestone or eggshells) at 44, 52, 60, 68, and 72 weeks of age. These eggs were obtained from an ongoing project to measure egg quality and dry matter content. Diet D1 showed higher albumen width and height, Haugh unit, and a lower egg yolk height than D2 (p2 than in D1. Other egg quality traits were similar between diets (p>0.05). The L2 was superior to L1 in both diets for egg quality traits (p2 in the extruded eggshell diet performed better than L2 in limestone diet. The egg, albumen, yolk, and eggshell weight, albumen width, and yolk height were increased but the eggshell strength, yolk color, albumen height, eggshell thickness, and membrane thickness were decreased with advancing the birds' age. Egg, albumen, yolk, and eggshell weight, as well as albumen width and yolk height, increased with the advancing age of the birds, whereas eggshell strength, yolk color, albumen height, eggshell thickness, and membrane thickness decreased. Egg, albumen, and eggshell with membrane weight were influenced by the diet × L interaction, while diet × age interaction affected other traits. Egg quality traits were influenced by L x A interaction, but were not affected by D x L x A interaction. The dry matter content of an egg was similar between diets, but moisture content was higher in D2 than in D1. The dry matter content of albumen was influenced by diet x age interaction. Therefore, the 8% extruded eggshell appears to be the most suitable diet for improving egg and eggshell quality.
},
year = {2025}
}
TY - JOUR T1 - Effect of Limestone or Extruded Eggshell on Egg and Eggshell Quality of Laying Hens AU - Mohammad Aminul Islam AU - Dibya Dipan Devnath AU - Israt Jahan Meem AU - Masahide Nishibori Y1 - 2025/04/29 PY - 2025 N1 - https://doi.org/10.11648/j.ijast.20250902.16 DO - 10.11648/j.ijast.20250902.16 T2 - International Journal of Animal Science and Technology JF - International Journal of Animal Science and Technology JO - International Journal of Animal Science and Technology SP - 74 EP - 86 PB - Science Publishing Group SN - 2640-1312 UR - https://doi.org/10.11648/j.ijast.20250902.16 AB - The study aimed to determine the effect of limestone or extruded eggshells on the egg and eggshell quality of laying hens. A total of 240 and 60 eggs were collected from diets D1 (limestone) and D2 (extruded eggshells), respectively, with two levels of calcium source (L): L1 (4% limestone or eggshells) and L2 (8% limestone or eggshells) at 44, 52, 60, 68, and 72 weeks of age. These eggs were obtained from an ongoing project to measure egg quality and dry matter content. Diet D1 showed higher albumen width and height, Haugh unit, and a lower egg yolk height than D2 (p2 than in D1. Other egg quality traits were similar between diets (p>0.05). The L2 was superior to L1 in both diets for egg quality traits (p2 in the extruded eggshell diet performed better than L2 in limestone diet. The egg, albumen, yolk, and eggshell weight, albumen width, and yolk height were increased but the eggshell strength, yolk color, albumen height, eggshell thickness, and membrane thickness were decreased with advancing the birds' age. Egg, albumen, yolk, and eggshell weight, as well as albumen width and yolk height, increased with the advancing age of the birds, whereas eggshell strength, yolk color, albumen height, eggshell thickness, and membrane thickness decreased. Egg, albumen, and eggshell with membrane weight were influenced by the diet × L interaction, while diet × age interaction affected other traits. Egg quality traits were influenced by L x A interaction, but were not affected by D x L x A interaction. The dry matter content of an egg was similar between diets, but moisture content was higher in D2 than in D1. The dry matter content of albumen was influenced by diet x age interaction. Therefore, the 8% extruded eggshell appears to be the most suitable diet for improving egg and eggshell quality. VL - 9 IS - 2 ER -
Department of Dairy and Poultry Science, Gazipur Agricultural University, Gazipur, Bangladesh
Department of Dairy and Poultry Science, Gazipur Agricultural University, Gazipur, Bangladesh
Department of Animal Breeding and Genetics, Gazipur Agricultural University, Gazipur, Bangladesh
Lab of Animal Genetics, Graduate School of Integrated Sciences for Life, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan
Information